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008 150904s2015 be a b 000 0 eng d
035 _a(ATU)b14876097
035 _a(OCoLC)988812042
040 _aATU
_beng
_erda
_cATU
082 0 4 _a637.1
_223
100 1 _aCasani, Sandra,
_eauthor.
245 1 0 _aInterlaboratory collaborative study on a flow cytometry method for lactic acid bacteria quantification in starter cultures, probiotics and fermented milk products according to ISO 19344/IDF232 /
_cSandra Casani, Ken Flemming Hansen and Stéphane Chartier.
264 1 _aBrussels, Belgium :
_bInternational Dairy Federation,
_c[2015]
264 4 _a©2015
300 _a49 pages :
_billustrations,
_c30 cm
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
338 _avolume
_2rdacarrier
490 1 _aBulletin of the International Dairy Federation ;
_v478/2015
520 _aAn international collaborative study of ISO 19344│IDF 232, Milk and milk products - Starter cultures, probiotics and fermented milk products - Quantification of lactic acid bacteria by flow cytometry was conducted to determine precision figures for the quantification of lactic acid bacteria and probiotic strains by flow cytometry in starter cultures, probiotics and fermented milk products. Except for yoghurts, the repeatability limit (r) and reproducibility limit (R) values were calculated to be 0.06 and 0.45 for log10 active fluorescent units (AFU) per gram and 0.07 and 0.38 for log10 total fluorescent units (TFU) per gram. The corresponding values for yoghurts were 0.08 and 0.76 for log10 AFU per gram and 0.10 and 1.17 for log10 TFU per gram. The latter reproducibility value illustrates the challenges encountered in analysis of TFU in yoghurts. Furthermore, equivalence of AFU results between the three different staining protocols was evaluated and demonstrated for most of the strains included in this study.
650 0 _aFermented milk.
_9337813
650 0 _aFlow cytometry
_xStandards
_9653198
650 0 _aBacterial starter cultures
_9375033
700 1 _aHansen, Flemming Ken,
_eauthor.
700 1 _aChartier, Stéphane,
_eauthor.
710 2 _aInternational Dairy Federation,
_eissuing body.
_9228132
830 0 _aBulletin of the International Dairy Federation ;
_v478/2015.
907 _a.b14876097
_b05-06-19
_c30-03-16
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_b01-04-16
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