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_cATU
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082 0 0 _a616.0277
_222
100 1 _aNg, Kee-Woei,
_eauthor.
_9834981
245 1 2 _aA manual for primary human cell culture /
_cKee Woei Ng, Jan-Thorsen Schantz.
246 1 1 _aPrimary human cell culture
250 _a2nd edition.
264 1 _aSingapore :
_bWorld Scientific,
_c[2010]
264 4 _c©2010
300 _axxiii, 188 pages :
_billustrations (some colour) ;
_c22 cm.
336 _atext
_btxt
_2rdacontent
337 _aunmediated
_bn
_2rdamedia
338 _avolume
_bnc
_2rdacarrier
490 1 _aManuals in biomedical research ;
_vv. 6
500 _aIncludes index.
505 0 0 _gA.
_tPrinciples of biosafety --
_gA.1.
_tCategories of biosafety --
_gA.2.
_tBiohazard materials --
_gA.3.
_tRecommended work practices --
_gA.4.
_tGeneral hygiene --
_gA.5.
_tHandling biological specimens --
_gB.
_tIntroduction to the cell culture laboratory --
_gB.1.
_tThe laminar flow workbench - biosafety cabinet --
_gB.2.
_tThe centrifuge --
_gB.3.
_tThe waterbath --
_gB.4.
_tThe sink/wet space --
_gB.5.
_tThe autoclave --
_gB.6.
_tThe refrigerator/freezer --
_gB.7.
_tThe incubator --
_gB.8.
_tThe microscope --
_gC.
_tProtocols for human cell culture --
_gC.1.
_tCell isolation --
_gC.2.
_tMesenchymal cells --
_gC.3.
_tEpithelial cells --
_gC.4.
_tBone marrow derived cells --
_gD.
_tReagents and techniques for cell culture --
_gD.1.
_tSelection of cell culture media --
_gD.2.
_tCell culture reagents and media supplements --
_gD.3.
_tCell dissociation products --
_gE.
_tTechniques for manipulating cell cultures and studying cellular behaviour --
_gE.1.
_tCryopreservation and thawing of cells --
_gE.2.
_tTransfection --
_gE.3.
_tQuantitative cell proliferation assays --
_gE.4.
_tAssessment of gene expression --
_gF.
_tTroubleshooting.
520 _aThis manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.--Back cover.
650 0 _aHuman cell culture
_vLaboratory manuals
_9628035
700 1 _aSchantz, Jan-Thorsten,
_eauthor.
_9262796
830 0 _aManuals in biomedical research ;
_vv. 6.
_9834975
907 _a.b13045957
_b11-07-17
_c28-10-15
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