TY  - BOOK
AU  - Casani,Sandra
AU  - Hansen,Flemming Ken
AU  - Chartier,Stéphane
ED  - International Dairy Federation,
TI  - Interlaboratory collaborative study on a flow cytometry method for lactic acid bacteria quantification in starter cultures, probiotics and fermented milk products according to ISO 19344/IDF232
T2  - Bulletin of the International Dairy Federation
U1  - 637.1 23
PY  - 2015///]
CY  - Brussels, Belgium
PB  - International Dairy Federation
KW  - Fermented milk
KW  - Flow cytometry
KW  - Standards
KW  - Bacterial starter cultures
N2  - An international collaborative study of ISO 19344│IDF 232, Milk and milk products  - Starter cultures, probiotics and fermented milk products - Quantification of lactic  acid bacteria by flow cytometry was conducted to determine precision figures for  the quantification of lactic acid bacteria and probiotic strains by flow cytometry in  starter cultures, probiotics and fermented milk products. Except for yoghurts, the  repeatability limit (r) and reproducibility limit (R) values were calculated to be 0.06  and 0.45 for log10 active fluorescent units (AFU) per gram and 0.07 and 0.38 for log10  total fluorescent units (TFU) per gram. The corresponding values for yoghurts were  0.08 and 0.76 for log10 AFU per gram and 0.10 and 1.17 for log10 TFU per gram. The  latter reproducibility value illustrates the challenges encountered in analysis of TFU  in yoghurts. Furthermore, equivalence of AFU results between the three different  staining protocols was evaluated and demonstrated for most of the strains included in  this study
ER  -