MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03272cam a2200397 i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20221102165057.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
130911t20102010si a 001 0|eng d |
| 011 ## - LINKING LIBRARY OF CONGRESS CONTROL NUMBER [OBSOLETE] |
|---|
| Local cataloguing issues note |
BIB MATCHES WORLDCAT |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
981283477X |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9789812834775 |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(ATU)b13045957 |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)687282347 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
ATU |
| Language of cataloging |
eng |
| Description conventions |
rda |
| Transcribing agency |
ATU |
| Modifying agency |
ATU |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
616.0277 |
| Edition number |
22 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ng, Kee-Woei, |
| Relator term |
author. |
| 9 (RLIN) |
834981 |
| 245 12 - TITLE STATEMENT |
|---|
| Title |
A manual for primary human cell culture / |
| Statement of responsibility, etc. |
Kee Woei Ng, Jan-Thorsen Schantz. |
| 246 11 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
Primary human cell culture |
| 250 ## - EDITION STATEMENT |
|---|
| Edition statement |
2nd edition. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Singapore : |
| Name of producer, publisher, distributor, manufacturer |
World Scientific, |
| Date of production, publication, distribution, manufacture, or copyright notice |
[2010] |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
©2010 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xxiii, 188 pages : |
| Other physical details |
illustrations (some colour) ; |
| Dimensions |
22 cm. |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Manuals in biomedical research ; |
| Volume/sequential designation |
v. 6 |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Includes index. |
| 505 00 - FORMATTED CONTENTS NOTE |
|---|
| Miscellaneous information |
A. |
| Title |
Principles of biosafety -- |
| Miscellaneous information |
A.1. |
| Title |
Categories of biosafety -- |
| Miscellaneous information |
A.2. |
| Title |
Biohazard materials -- |
| Miscellaneous information |
A.3. |
| Title |
Recommended work practices -- |
| Miscellaneous information |
A.4. |
| Title |
General hygiene -- |
| Miscellaneous information |
A.5. |
| Title |
Handling biological specimens -- |
| Miscellaneous information |
B. |
| Title |
Introduction to the cell culture laboratory -- |
| Miscellaneous information |
B.1. |
| Title |
The laminar flow workbench - biosafety cabinet -- |
| Miscellaneous information |
B.2. |
| Title |
The centrifuge -- |
| Miscellaneous information |
B.3. |
| Title |
The waterbath -- |
| Miscellaneous information |
B.4. |
| Title |
The sink/wet space -- |
| Miscellaneous information |
B.5. |
| Title |
The autoclave -- |
| Miscellaneous information |
B.6. |
| Title |
The refrigerator/freezer -- |
| Miscellaneous information |
B.7. |
| Title |
The incubator -- |
| Miscellaneous information |
B.8. |
| Title |
The microscope -- |
| Miscellaneous information |
C. |
| Title |
Protocols for human cell culture -- |
| Miscellaneous information |
C.1. |
| Title |
Cell isolation -- |
| Miscellaneous information |
C.2. |
| Title |
Mesenchymal cells -- |
| Miscellaneous information |
C.3. |
| Title |
Epithelial cells -- |
| Miscellaneous information |
C.4. |
| Title |
Bone marrow derived cells -- |
| Miscellaneous information |
D. |
| Title |
Reagents and techniques for cell culture -- |
| Miscellaneous information |
D.1. |
| Title |
Selection of cell culture media -- |
| Miscellaneous information |
D.2. |
| Title |
Cell culture reagents and media supplements -- |
| Miscellaneous information |
D.3. |
| Title |
Cell dissociation products -- |
| Miscellaneous information |
E. |
| Title |
Techniques for manipulating cell cultures and studying cellular behaviour -- |
| Miscellaneous information |
E.1. |
| Title |
Cryopreservation and thawing of cells -- |
| Miscellaneous information |
E.2. |
| Title |
Transfection -- |
| Miscellaneous information |
E.3. |
| Title |
Quantitative cell proliferation assays -- |
| Miscellaneous information |
E.4. |
| Title |
Assessment of gene expression -- |
| Miscellaneous information |
F. |
| Title |
Troubleshooting. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.--Back cover. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Human cell culture |
| Form subdivision |
Laboratory manuals |
| 9 (RLIN) |
628035 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Schantz, Jan-Thorsten, |
| Relator term |
author. |
| 9 (RLIN) |
262796 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
Manuals in biomedical research ; |
| Volume/sequential designation |
v. 6. |
| 9 (RLIN) |
834975 |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) |
|---|
| a |
.b13045957 |
| b |
11-07-17 |
| c |
28-10-15 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Book |
| 945 ## - LOCAL PROCESSING INFORMATION (OCLC) |
|---|
| a |
616.0277 NG |
| g |
1 |
| i |
A518392B |
| j |
0 |
| l |
cmain |
| o |
- |
| p |
$43.66 |
| q |
- |
| r |
- |
| s |
- |
| t |
0 |
| u |
1 |
| v |
0 |
| w |
0 |
| x |
0 |
| y |
.i13353408 |
| z |
29-10-15 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) |
|---|
| -- |
(2)b |
| -- |
(2)c |
| Operator's initials, OID (RLIN) |
06-04-16 |
| Cataloger's initials, CIN (RLIN) |
m |
| First date, FD (RLIN) |
a |
| -- |
eng |
| -- |
si |
| -- |
2 |
